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Permeabilization of Escherichia coli cells for enhanced penicillin acylase activity

✍ Scribed by V. Nagalakshmi; J. S. Pai

Publisher: Springer-Verlag
Year: 1994
Tongue: English
Weight: 227 KB
Volume: 8
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00154316

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✦ Synopsis

Escherichia coli cells with penicillin acylase activity were permeabilized with aqueous solutions of the cationic detergent N-cetyl-N,N,N-trimethylammonium bromide (CTAB), at pH 8.0 and the activity was found to have almost doubled. The concentration of CTAB, the time and temperature of treatment were optimised for maximum enzyme activity and were found to be 0.2%, 20 min and 5'C respectively. Subsequently, the cell bound activity was retained for a longer period by chemical cross-linking with 0.1% glutaraldehyde.

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