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Enhancement of penicillin acylase activity by cultivating immobilizedKluyvera citrophila

✍ Scribed by Yasushi Morikawa; Isao Karube; Shuichi Suzuki

Publisher
Springer
Year
1980
Tongue
English
Weight
399 KB
Volume
10
Category
Article
ISSN
1432-0614

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✦ Synopsis

Whole cells of Kluyvera citropbila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%-70% of native cells. When the immobilized cells were continuously cultivated for 40 h in an aerated fermentor containing peptone medium and were treated with alkali in order to remove/3-1actamase activity, the immobilized cells produced ampicillin up to 4.4 times faster than noncultivated cells.

Ampicillin production was investigated in a column system using these cultivated immobilized whole ceils. The cultivated immobilized cells showed excellent performance in continuous ampicillin production.

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