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Stabilization of heterodimeric enzyme by multipoint covalent immobilization: Penicillin G acylase from Kluyvera citrophila

✍ Scribed by José M. Guisán; Gregorio Alvaro; Roberto Fernandez-Lafuente; Cristina M. Rosell; Jose L. Garcia; Auro Tagliani


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
1001 KB
Volume
42
Category
Article
ISSN
0006-3592

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✍ Jesús Torres-Bacete; Miguel Arroyo; Raquel Torres-Guzmán; Isabel de la Mata; M P 📂 Article 📅 2001 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 103 KB

## Abstract Penicillin V acylase from the actinomycete __Streptomyces lavendulae__ ATCC 13664 has been immobilized to epoxy‐activated acrylic beads (Eupergit C®) by covalent binding. Further linkage of bovine serum albumin after enzyme immobilization was carried out in order to remove the remaining