## Abstract The recovery of penicillin acylase from __E coli__ by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the per
Enhancement of the enantioselectivity of penicillin G acylase from E. coli by “substrate tuning”
✍ Scribed by Torsten Pohl; Herbert Waldmann
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- French
- Weight
- 261 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0040-4039
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Several techniques for protein extraction were tested for recovering penicillin acylase from a recombinant strain of Escherichia coli. These techniques include chemical [guanidine hydrochloride, Triton X-100, ethylenediaminetetraacetic acid (EDTA), ethanol/toluene], physical (sonication, freeze-and-