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Selective reverse micellar extraction of penicillin acylase from E coli

✍ Scribed by Vilas G Gaikar; Madhav S Kulkarni

Publisher
Wiley (John Wiley & Sons)
Year
2001
Tongue
English
Weight
145 KB
Volume
76
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

The recovery of penicillin acylase from E coli by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the periplasmic space of E coli without disrupting the cells. Unlike ultrasonication which breaks open the cells entirely, making subsequent processing difficult and expensive, reverse micellar treatment of cells gave a moderate recovery of 60% of enzyme activity in a highly pure form.

Β© 2001 Society of Chemical Industry

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Several techniques for protein extraction were tested for recovering penicillin acylase from a recombinant strain of Escherichia coli. These techniques include chemical [guanidine hydrochloride, Triton X-100, ethylenediaminetetraacetic acid (EDTA), ethanol/toluene], physical (sonication, freeze-and-