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Factors affecting the synthesis of penicillins by the immobilized penicillin acylase from Escherichia coli

โœ Scribed by K. P. Koteva; K. D. Ganchev

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
350 KB
Volume
14
Category
Article
ISSN
0138-4988

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## Abstract Highโ€level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation