Electrohydrodynamic Comminution: A Novel Technique for the Aerosolisation of Plasmid DNA
โ Scribed by Lee A. Davies; Kevin Hannavy; Neville Davies; Alistair Pirrie; Ronald A. Coffee; Stephen C. Hyde; Deborah R. Gill
- Book ID
- 106492587
- Publisher
- Springer US
- Year
- 2005
- Tongue
- English
- Weight
- 468 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0724-8741
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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif
A rapid method is described for the isolation of plasmid DNA from Escherichia coli and Pseudomonas putida. The effect of heating the cell preparation during plasmid extraction is discussed in relationship to the final plasmid yield.