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A plasmid rescue technique for the recovery of plant DNA disrupted by T-DNA insertion

✍ Scribed by Friedrich J. Behringer; June I. Medford


Book ID
112821078
Publisher
Springer
Year
1992
Tongue
English
Weight
654 KB
Volume
10
Category
Article
ISSN
0735-9640

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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif