A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif
โฆ LIBER โฆ
A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA
โ Scribed by Hideaki Hashimoto; Hiromichi Morikawa; Yasuyuki Yamada; Akira Kimura
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 300 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1432-0614
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