A rapid and reliable method for PCR-based amplification of chromosomal and mitochondrial DNA from intact yeast cells
โ Scribed by R. Jesnowski; J. Naehring; K. Wolf
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 197 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif
Mutations in the pet18 gene of Saccharomyces cerevisiae (formerly denoted pets) confer three phenotypes on mutant strains: (i) inability to respire (petite), (ii) inability to maintain the double-stranded RNA killer plasmid (sensitive), and (iii) temperature sensitivity for growth. We find that pet1