✦ LIBER ✦

A rapid method for the isolation of plasmid DNA from bacteria

✍ Scribed by J. T. Trevors

Publisher: Springer Netherlands
Year: 1984
Tongue: English
Weight: 286 KB
Volume: 6
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129309

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid method is described for the isolation of plasmid DNA from Escherichia coli and Pseudomonas putida. The effect of heating the cell preparation during plasmid extraction is discussed in relationship to the final plasmid yield.

📜 SIMILAR VOLUMES

A rapid and simplified protocol for DNA

A rapid and simplified protocol for DNA isolation from bacteria

✍ D. L. Troyer; A. Reed; D. Oberst; M. M. Chengappa 📂 Article 📅 1990 🏛 Springer Netherlands 🌐 English ⚖ 711 KB

Direct Lysis Method for the Rapid Prepar

Direct Lysis Method for the Rapid Preparation of Plasmid DNA

✍ Jun Song; Christian Geltinger; Kailai Sun; Ichirou Kanazawa; Kazunari K. Yokoyam 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 123 KB

Mini mu containing plasmid and a method

Mini mu containing plasmid and a method for rapid dna sequencing

📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 116 KB

A rapid inexpensive method for the isola

A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources

✍ Albert J. Wilson; Prem S. Chourey 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 459 KB

A procedure for the isolation and purifi

A procedure for the isolation and purification of plasmid DNA from Rhizobium meliloti

✍ T. Adachi; V.N. Iyer 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 710 KB

A procedure is described for the isolation and purification of the DNA of plasmids that are indigenous to the agriculturally important nitrogen-fixing bacterium Rhizobium meliloti.

A simple procedure for large-scale prepa

A simple procedure for large-scale preparation of pure plasmid DNA free from chromosomal DNA from bacteria

✍ M. Mukhopadhyay; N.C. Mandal 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 870 KB

A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu