Effect of polymer structure on affinity partitioning of lactate dehydrogenase in polymer—water two-phase systems

✍ Scribed by Göte Johansson; Monica Joelsson

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 428 KB
• Volume: 411
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93967-0

✦ Synopsis

Lactate dehydrogenase from rabbit muscle (EC 1.1.1.27) was partitioned between the liquid phases of aqueous two-phase systems containing two kinds of polymers. The partition was influenced by the use of a dye ligand, Procion Yellow HE-3G, bound to a polymer. The ligand-carrying polymers were poly(ethyleneglycol), polyjvinyl alcohol). ethylhydroxyethylcellulose, hydroxypropyl-starch, Ficoll and dextran. Their influence on the partition of lactate dehydrogenase was studied in systems composed of the following pairs of polymers: dextran-poly(ethylene glycol), dextran-poly(viny1 alcohol), dextran-Ficoll and hydroxypropyl-starch-poly(ethylene glycol). The ratio between the change in the logarithmic partition coefficient of the enzyme, A log K, caused by the ligand and the logarithm of the partition coefficient of the ligand-polymer, log KL_-polynrcr, was used as a measure of the number of ligand polymers bound per enzyme molecule under saturation conditions. Possible explanations for the observed effects are discussed.