✦ LIBER ✦

Combination of polymer-bound charged groups and affinity ligands for extraction of enzymes by partitioning in aqueous two-phase systems

✍ Scribed by Lüling Cheng; Monica Joelsson; Göte Johansson

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 751 KB
Volume: 523
Category: Article
ISSN: 1873-3778
DOI: 10.1016/0021-9673(90)85016-o

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Affinity partitioning of enzymes in aque

Affinity partitioning of enzymes in aqueous two-phase systems containing dyes and their copper(II) complexes bound to poly(ethylene glycol)

✍ Vilius Žutautas; Birute Baškevičiūte; Henrikas Pesliakas 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 951 KB

Surface charge and hydrophobic propertie

Surface charge and hydrophobic properties of fresh and cryopreserved blood phagocytes as determined by partition in two-phase aqueous polymer systems

✍ Dr. R. S. Hill; R. Norris-Jones; B. Still; D. E. Brooks 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 527 KB

Synthesis and application of a poly(ethy

Synthesis and application of a poly(ethylene glycol)-antibody affinity ligand for cell separations in aqueous polymer two-phase systems

✍ Kim A. Sharp; Manssur Yalpani; Stephen J. Howard; Donald E. Brooks 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 655 KB

The possibility of producing biospecific affinity ligands for separating cells in two polymer aqueous phase systems on the basis of cell surface antigens was investigated. Rabbit anti-human erythrocyte IgG was reacted with cyanuric chloride-activated monomethyl poly(ethylene glycol) (PEG) fractions

Use of aqueous two-phase systems for in

Use of aqueous two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports

✍ Odette Hernandez-Justiz; Roberto Fernandez-Lafuente; Marco Terreni; Jose M. Guis 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 333 KB 👁 2 views

Yields of kinetically controlled synthesis of antibiotics catalyzed by penicillin G acylase from Escherichia coli (PGA) have been greatly increased by con- tinuous extraction of water soluble products (cephalexin) away from the surroundings of the enzyme. In this way its very rapid enzymatic hydroly

Partition of free and monoclonal-antibod

Partition of free and monoclonal-antibody-bound horseradish peroxidase in a two-phase aqueous polymer system—Novel procedure for the determination of the apparent binding constant of monoclonal antibody to horseradish peroxidase

✍ L. Elling; M.-R. Kula; E. Hadas; E. Katchalski-Katzir 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 415 KB

The principle that the antigen and the antibody prefer different phases in an aqueous two-phase system is the analytical basis of the work presented here. The antigen horseradish peroxidase, which is bound to a monoclonal antibody (mAb), is separated from free Ag in an aqueous phase system (polyethy