Electrostatic Effects on Partitioning of Proteins in Aqueous Two-Phase Systems: II. Charged Polymers
โ Scribed by Moon-Sun Lin; P. Neogi
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 524 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0021-9797
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๐ SIMILAR VOLUMES
Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating
Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.