Electrostatic Effects on Partitioning of Proteins in Aqueous Two-Phase Systems: I. pH and Charge Equilibria
โ Scribed by P. Neogi
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 672 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0021-9797
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๐ SIMILAR VOLUMES
Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating
## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous twoโphase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig