Genetically engineered charge modifications to enhance protein separation in aqueous two-phase systems: Charge directed partitioning
β Scribed by John R. Luther; Charles E. Glatz
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 665 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0006-3592
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π SIMILAR VOLUMES
Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating
Genetic engineering has been used for the fusion of peptides, with different length and composition, on a protein to study the effect on partitioning in aqueous two-phase systems containing thermoseparating polymers. Peptides containing 2-6 tryptophan residues or tryptophan plus 1-3 lysine or aspart
## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous twoβphase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig