Effect of antioxidants on cultured human diploid fibroblasts exposed to cystine-free medium
โ Scribed by Shiro Bannai; Hohko Tsukeda; Hideo Okumura
- Book ID
- 118311555
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 353 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
## Abstract Human diploid fibroblasts take up cystine in the culture medium and the cystine is immediately reduced to cysteine in the cells. It is found that cysteine thus formed is rapidly released from the cells into the medium and accumulates there. The system transporting cysteine is convincing
Two neoplastic human cell lines, WI-38 CT-I and SUSM-I, which were transformed in vitmwith gamma rays and 4 nitroquinoline I oxide, respectively, grew continuously in a serum-free defined medium. The defined medium used was a I:I mixture by volume of Dulbecco's modified