## Abstract We have developed two serumβfree chemically defined media (RITC 78β6 and RITC 80β7) that support the growth in culture of human diploid fibroblasts to the same extent as Eagle's basal medium (BME) supplemented with 10% fetal bovine serum (FBS). These two media contain modified Eagle's m
Effects of ferrous iron and transferrin on cell proliferation of human diploid fibroblasts in serum-free culture
β Scribed by Mikio Kan; Isao Yamane
- Book ID
- 112800693
- Publisher
- Society for In Vitro Biology
- Year
- 1984
- Tongue
- English
- Weight
- 551 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1475-2689
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π SIMILAR VOLUMES
Two neoplastic human cell lines, WI-38 CT-I and SUSM-I, which were transformed in vitmwith gamma rays and 4 nitroquinoline I oxide, respectively, grew continuously in a serum-free defined medium. The defined medium used was a I:I mixture by volume of Dulbecco's modified
## Abstract Human neonatal skin fibroblasts plated sparsely in MCDB 105 traversed a complete cell cycle in the absense of serum or serumβderived proteins. Addition of pure PDGF did not significantly increase entrance into S phase as revealed by ^3^Hβthymidine labeling index or clonal growth on pall