## Abstract We have developed two serumโfree chemically defined media (RITC 78โ6 and RITC 80โ7) that support the growth in culture of human diploid fibroblasts to the same extent as Eagle's basal medium (BME) supplemented with 10% fetal bovine serum (FBS). These two media contain modified Eagle's m
Differential growth response of normal human diploid fibroblasts and in vitro transformed human fibroblasts in serum-free defined culture medium
โ Scribed by Masayoshi Namba; Takashi Kusaka; Fujiko Fukushima; Tetsuo Kimoto
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 554 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0020-7136
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โฆ Synopsis
Two neoplastic human cell lines, WI-38 CT-I and SUSM-I, which were transformed in vitmwith gamma rays and 4 nitroquinoline I oxide, respectively, grew continuously in a serum-free defined medium. The defined medium used was a I:I mixture by volume of Dulbecco's modified
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## Abstract The hormones which support growth, in vitro, of normal, neonatal human foreskin fibroblasts were determined. Wheresas thrombin and hydrocortisone were major growth stimulants, plateletโderived growth factor was not. Human foreskin fibroblasts grew in a serumโfree, biochemically defined
## Abstract Marineโderived collagen is expected to be a much safer alternative to calf collagen, which in medical applications carries the risk of bovine spongiform encephalopathy. In this study, acidโsoluble collagen was extracted from salmon skin and crosslinked with 1โethylโ3โ(3โdimethylaminopro
The growth regulation of human diploid fibroblasts by platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin-like growth factor I (IGF-I) (somatomedin C), dexamethasone, and transferrin was investigated in a serumfree, chemically defined culture system. Cell-cycle kinetic para
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