Effects of cystine or glutamine restriction on human diploid fibroblasts in culture
โ Scribed by Valerie Ann Corfield; Robert J. Hay
- Book ID
- 112797655
- Publisher
- Society for In Vitro Biology
- Year
- 1978
- Tongue
- English
- Weight
- 654 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1475-2689
No coin nor oath required. For personal study only.
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## Abstract Human diploid fibroblasts take up cystine in the culture medium and the cystine is immediately reduced to cysteine in the cells. It is found that cysteine thus formed is rapidly released from the cells into the medium and accumulates there. The system transporting cysteine is convincing
Histone variant synthesis patterns from human diploid fibrobladike cells of different in vitro ages were determined during exponential growth, at confluence, and during low serum arrest. The results are reported as the ratios of H2A variant synthesis (H2A.1 and HZA.2iH2A.x and H2A.z) and H3 variant