The influence of culture medium volume on cell density and lifespan of human diploid fibroblasts
โ Scribed by J.M. Ryan; B.B. Sharf; V.J. Cristofalo
- Book ID
- 118876625
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 299 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0014-4827
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract When human diploid fibroblasts IMRโ90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium. The major component of these sulfhydryl compounds is cysteine, and it is shown that a part of medium cyst
In order to analyze changes in metabolism of insulin-like growth factor binding proteins (IGFBPs) related to cell senescence and cell density, we compared human diploid fibroblasts (HDF) in the proliferatively vigorous first half (young cells) and senescent HDF in the last 10% (old cells) of the rep