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Formation of sulfhydryl groups in the culture medium by human diploid fibroblasts

✍ Scribed by Shiro Bannai; Tetsuro Ishii

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
696 KB
Volume
104
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

When human diploid fibroblasts IMR‐90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium. The major component of these sulfhydryl compounds is cysteine, and it is shown that a part of medium cystine is converted into cysteine by the cells. It is also shown that the sulfhydryl groups of serum albumin, which are masked and barely detectable before the culture, are restored. Probably cysteine formed by the cells reacts with serum albumin to give rise to the protein sulfhydryl groups via sulfhydryl–disulfide exchange reactions. Total sulfhydryl concentrations in the medium are maintained in a considerable level throughout the culture, and a possible physiological function of these sulfhydryl groups is discussed.

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