✦ LIBER ✦

Selective inhibition of the cell cycle of cultured human diploid fibroblasts by aminonucleoside of puromycin

✍ Scribed by George P. Studzinski; John F. Gierthy

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 933 KB
Volume: 81
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040810109

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✦ Synopsis

Abstract

Aminonucleoside (AMS) inhibited the cell cycle of human lung fibroblasts at a point in G1 phase and at another point in G2 phase. Even when this inhibition was fully established, DNA synthesis and mitosis which were in progress proceeded normally. Inhibition of RNA synthesis in the cultures preceded the effects on DNA synthesis and mitosis, but inhibition of protein synthesis could not be detected.

These points of potential inhibition do not exist in the cell cycle of HeLa cell, or are not affected by aminonucleoside. Here inhibition of cell proliferation by AMS was less marked, and when inhibition eventually occurred it was not specific for any point of the cell cycle. The rate of entry of the inhibitor was similar in both types of cell.

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