𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone

✍ Scribed by Gary L. Grove; Vincent J. Cristofalo

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
599 KB
Volume
90
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Age‐related changes in the cytokinetics of human diploid cells in vitro have been compared in normal cultures and in cultures in which lifespan has been prolonged by the addition of hydrocortisone to the medium. For both cultures, with advancing age the fraction of cells in the actively proliferating pool decreased and the intercellular variation in cell cycle times increased. The average cell cycle time was prolonged during aging due almost entirely to changes in the duration of G~1~. The duration of S remained constant, while a small delay in G~2~ was observed in late passage cells near the end of their lifespan. Although the same pattern of change in proliferative parameters occurred in both control and hydrocortisone‐treated cultures, the changes were somewhat delayed in the presence of the steroid. The results are interpreted in terms of several cell cycle models and suggest that the events controlling cell proliferation are sensitive to hydrocortisone modulation during the G~1~ and possibly the G~2~ periods.

📜 SIMILAR VOLUMES

Selective inhibition of the cell cycle o
Selective inhibition of the cell cycle of cultured human diploid fibroblasts by aminonucleoside of puromycin
✍ George P. Studzinski; John F. Gierthy 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 933 KB

## Abstract Aminonucleoside (AMS) inhibited the cell cycle of human lung fibroblasts at a point in G1 phase and at another point in G2 phase. Even when this inhibition was fully established, DNA synthesis and mitosis which were in progress proceeded normally. Inhibition of RNA synthesis in the cult

Superoxide dismutase specific activities
Superoxide dismutase specific activities in cultured human diploid cells of various donor ages
✍ Michael R. Duncan; Robert T. Dell'orco; K. D. Kirk 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 383 KB 👁 2 views

## Abstract It has been postulated that superoxide dismutase (SOD) protects cells from free radical‐induced damage. In these experiments SOD specific activity was measured as established human diploid cell lines from various donor ages progressed through their in vitro lifespans. Significant elevat

Cell cycle effects of hypertonic stress
Cell cycle effects of hypertonic stress on various human cells in culture
✍ C. Pellicciari; C. Filippini; L. De Grada; A. M. Fuhrman Conti; M. G. Manfredi R 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 639 KB

## Abstract Long‐term exposure to hypertonic (HT) culture media has been found to perturb the cell cycle and change gene expression in various animal cell types. A lower growth rate, with exit of cells from the cycling compartment has been observed previously in human transformed EUE. cells. The ai

Proliferative capacity and DNA content o
Proliferative capacity and DNA content of aging human diploid cells in culture: A cytophotometric and autoradiographic analysis
✍ Rosalind Yanishevsky; Mortimer L. Mendelsohn; Brian H. Mayall; Dr Vincent J. Cri 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 394 KB 👁 2 views

## Abstract Using a combination of DNA‐cytophotometry and tritiated thymidine‐autoradiography, we have shown that the majority of nondividing cells in serially propagated human diploid cell populations have the 2C DNA content consistent with their being arrested in the G~1~ phase of the diploid cel

Transport of cystine and cysteine and ce
Transport of cystine and cysteine and cell growth in cultured human diploid fibroblasts: Effect of glutamate and homocysteate
✍ Shiro Bannai; Tetsuro Ishii 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 901 KB

## Abstract Human diploid fibroblasts take up cystine in the culture medium and the cystine is immediately reduced to cysteine in the cells. It is found that cysteine thus formed is rapidly released from the cells into the medium and accumulates there. The system transporting cysteine is convincing

Expression of cell growth and bone pheno
Expression of cell growth and bone phenotypic genes during the cell cycle of normal diploid osteoblasts and osteosarcoma cells
✍ Laura R. McCabe; T. J. Last; Maureen Lynch; Jane Lian; Janet Stein; Gary Stein 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 740 KB

## Abstract Establishing reuglatory mechanisms that mediate proliferation of osteoblasts while restricting expression of genes asociated with mature bone cell phenotypic properties to post‐proliferative cells is fundamental to understanding skeletal development. To gain insight into relationships b