## Abstract Affinity chromatography of proteins requires a ligand covalently bound to a solid support separated by a spacer of sufficient length. In the specific case of acetylβcholinesterase we have reduced the conventional spacer synthesis from five to three steps. For affinity chromatography of
β¦ LIBER β¦
Discovery and design of affinity ligands for protein chromatography
β Scribed by Liu, Fu-Feng; Dong, Xiao-Yan; Sun, Yan
- Book ID
- 122372268
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 68 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0168-1656
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## Abstract Bifunctional affinity ligands based on a triazine scaffold were rationally designed to target prion protein and shown to bind recombinant prion protein with high affinity and selectivity. The ligands were capable of discriminating between prion protein glycoforms and monomeric and dimer