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Direct Electroporation of DNA from In-Gel Ligation Mixtures

โœ Scribed by R.L. Shattuck; S.P. Register


Book ID
102558803
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
265 KB
Volume
230
Category
Article
ISSN
0003-2697

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Direct hybridization of labeled DNA to D
โœ M. Purrello; I. Balazs ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 989 KB

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive pro