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Preparation of complementary dna by direct synthesis on plant virus RNAs from agarose gels

โœ Scribed by D. Gallitelli; R. Hull


Book ID
119114243
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
297 KB
Volume
11
Category
Article
ISSN
0166-0934

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A method is described which allows the obtention of total DNA from cells of Chlamydomonas reinhardi in 1 day. It is based on the gel filtration on agarose of crude lysates incubated for 1 hr with pronase and subsequently brought to 2 M NaCl in order to dissociate nucleoprotein complexes. The DNA ob