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Dependence of the Ligation Efficiency of Large DNA Fragments Isolated from Agarose Gels on the Purification Method

โœ Scribed by Fransen, Erik; Camp, Guy Van; Winnepenninckx, Birgitta

Book ID
121003150
Publisher
Taylor and Francis Group
Year
1998
Tongue
English
Weight
335 KB
Volume
28
Category
Article
ISSN
1082-6068

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A procedure for quick and simple elution of DNA from agarose gels is presented. After electrophoresis, bands of interest are cut out of the gel and the slices are equilibrated in a neutral salt buffer. The slices are then frozen and centrifuged through a filtration assembly whereby the DNA-containin