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Direct hybridization of labeled DNA to DNA in agarose gels

โœ Scribed by M. Purrello; I. Balazs

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
989 KB
Volume
128
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive probe, and the hybridization pattern visualized by autoradiography. This method is sensitive enough to detect single or low copy number DNA sequences in the genome of higher eucaryotes.

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