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Improved recovery of DNA from polyacrylamide gels after in situ DNA footprinting

✍ Scribed by Geertje van Keulen; Wim G. Meijer

Book ID: 117360133
Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 122 KB
Volume: 54
Category: Article
ISSN: 0167-7012
DOI: 10.1016/s0167-7012(03)00045-9

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %