Improved conditions for silver–ammonia staining of DNA in polyacrylamide gel
✍ Scribed by Wei-Tao Cong; Hong-Zhang He; Zhong-Xin Zhu; Cai-Xue Ye; Xu-Yi Yang; Jung-Kap Choi; Li-Tai Jin; Xiao-Kun Li
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 134 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0173-0835
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📜 SIMILAR VOLUMES
A method for detection of glycoproteins in thin sodium dodecyl sulfate polyacrylamide gels was developed by a combination of (i) initial periodic acid oxidation/Alcian blue staining and (ii) subsequent staining with silver nitrate. The procedure allowed detection of as little as \(1.6 \mathrm{ng}\)
## Abstract A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm^2^). Sensitivity of detection is superior to ethidium bromide and, for 11 double‐stranded fragments of ϕX174 DNA, staining is linear with respect to concen