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Improved conditions for silver–ammonia staining of DNA in polyacrylamide gel

✍ Scribed by Wei-Tao Cong; Hong-Zhang He; Zhong-Xin Zhu; Cai-Xue Ye; Xu-Yi Yang; Jung-Kap Choi; Li-Tai Jin; Xiao-Kun Li


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
134 KB
Volume
31
Category
Article
ISSN
0173-0835

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Improved Method for Silver Staining of G
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A method for detection of glycoproteins in thin sodium dodecyl sulfate polyacrylamide gels was developed by a combination of (i) initial periodic acid oxidation/Alcian blue staining and (ii) subsequent staining with silver nitrate. The procedure allowed detection of as little as \(1.6 \mathrm{ng}\)

Silver staining of DNA in polyacrylamide
✍ David Goldman; Dr. Carl R. Merril 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 280 KB

## Abstract A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm^2^). Sensitivity of detection is superior to ethidium bromide and, for 11 double‐stranded fragments of ϕX174 DNA, staining is linear with respect to concen