An optimal method of DNA silver staining in polyacrylamide gels
β Scribed by Yan-Chuang Han; Cai-Zhu Teng; Zhong-Li Hu; Yun-Chun Song
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 254 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
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## Abstract A sensitive silver staining method using eriochrome blackβ T as a silverβion sensitizer for DNA detection in polyacrylamide gels was developed. The sensitivity of this staining method was significantly improved by the new silverβion sensitizer containing a diazo group, which has reducing
## Abstract A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm^2^). Sensitivity of detection is superior to ethidium bromide and, for 11 doubleβstranded fragments of ΟX174 DNA, staining is linear with respect to concen