Silver staining method for DNA in polyacrylamide gels using eriochrome black T as a silver-ion sensitizer

✍ Scribed by Sun-young Hwang; Li-tai Jin; Gyurng-soo Yoo; Jung-Kap Choi

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 202 KB
• Volume: 27
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200500601

✦ Synopsis

Abstract

A sensitive silver staining method using eriochrome black T as a silver‐ion sensitizer for DNA detection in polyacrylamide gels was developed. The sensitivity of this staining method was significantly improved by the new silver‐ion sensitizer containing a diazo group, which has reducing power. The staining method lasted a total of approximately 15 min following a fixing step for 2×20 min. The detection limit of this staining method was 1–4 pg for ΦX174 DNA/__Hae__III in both nondenaturing and denaturing polyacrylamide gels. This staining method was especially effective in low‐base pair DNA, with a sensitivity that was approximately ten‐fold higher than previously published silver staining methods.