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Development of a high-throughput screening method for recombinantEscherichia coliwith intracellular dextransucrase activity

✍ Scribed by So-Ra Lee; Ah-Rum Yi; Hong-Gyun Lee; Myoung-Uoon Jang; Jung-Mi Park; Nam Soo Han; Tae-Jip Kim


Book ID
107643288
Publisher
The Microbiological Society of Korea
Year
2011
Tongue
English
Weight
233 KB
Volume
49
Category
Article
ISSN
1225-8873

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About 1000 catalytic or stoichiometric asymmetric reactions of racemic compounds or prochiral substrates bearing enantiotopic groups can be analyzed per day. In this highly efficient method the enantioselectivity is determined by electrospray ionization mass spectrometry using isotopically labeled s