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A new high-throughput screening method for determining active and enantioselective hydrolases

✍ Scribed by Bo Wang; Xiaoling Tang; Gangfeng Ren; Ji Liu; Hongwei Yu


Book ID
108068860
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
834 KB
Volume
46
Category
Article
ISSN
1369-703X

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About 1000 catalytic or stoichiometric asymmetric reactions of racemic compounds or prochiral substrates bearing enantiotopic groups can be analyzed per day. In this highly efficient method the enantioselectivity is determined by electrospray ionization mass spectrometry using isotopically labeled s