A High-Throughput-Screening Method for the Identification of Active and Enantioselective Hydrolases
✍ Scribed by U. T. Bornscheuer; R. Stürmer; M. Baumann
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- German
- Weight
- 31 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0009-286X
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📜 SIMILAR VOLUMES
About 1000 catalytic or stoichiometric asymmetric reactions of racemic compounds or prochiral substrates bearing enantiotopic groups can be analyzed per day. In this highly efficient method the enantioselectivity is determined by electrospray ionization mass spectrometry using isotopically labeled s
Two NMR-based approaches for highthroughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo-enantiomers or pseudo-meso-compounds based on 13 C-labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate