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A Practical NMR-Based High-Throughput Assay for Screening Enantioselective Catalysts and Biocatalysts

✍ Scribed by Manfred T. Reetz; Andreas Eipper; Patrick Tielmann; Richard Mynott

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
148 KB
Volume
344
Category
Article
ISSN
1615-4150

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✦ Synopsis

Two NMR-based approaches for highthroughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo-enantiomers or pseudo-meso-compounds based on 13 C-labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate flow-through cell and an autosampler. The other approach is based on tradi-tional diastereomer formation using a chiral reagent or complexing agent. The ee values are accurate to within AE 2% and AE 5% of the true values.

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