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Determination of the structure of the capsular antigen of Escherichia coli O8:K46:H30, using FABMS and 2D-NMR spectroscopy

✍ Scribed by Guy G.S. Dutton; Agyeman Kuma-Mintah; Stephen K. Ng; Haralambos Parolis; Anne Dell; Andrew Reason

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 676 KB
Volume: 231
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(92)84005-d

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✦ Synopsis

The structure of the capsular antigen from Escherichia coli O8:K46:H30 was elucidated by methylation analysis and 1D and 2D 1H- and 13C-NMR spectroscopy, and by methylation analysis, 1D- and 2D-NMR spectroscopy, and FABMS of the oligosaccharide-alditol obtained after dephosphorylation of the polymer with aqueous hydrofluoric acid. The capsular polymer is of the teichoic acid type and has the following repeating unit. [Formula: see text]

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