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Structural determination of the capsular antigen of Escherichia coli O8:K25:H9

✍ Scribed by A.Nixon Anderson; Lesley A.S. Parolis; Haralambos Parolis

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 478 KB
Volume: 265
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)00209-6

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✦ Synopsis

The primary structure of the acidic capsular antigen of Escherichia coli 08:K25:H9 was shown by monosaccharide analysis, methyl&on analysis, and by 1D and 2D 'H and 13C NMR spectroscopy to be composed of linear tetrasaccharide repeating units having the structure: + 2) -fi-D-GkpA-( 1 --, 3) -cY-D-GalpNAc-( 1 + 3)-CY-o-GalpNAc-( 1 -+ 2)-c~-D-Fucp-( 1 --, Keywordx Escherichia coli; Capsulru polysaccharide; K25 antigen; NMR spectroscopy Isolation, composition, and methylation analysis.-E. coli 08:K25:H9

bacteria were grown on Mueller-Hinton agar (see Experimental section), the capsular polysaccharide was extracted from the cells with aqueous 1% phenol, and was purified by precipitation

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