The primary structure of the acidic capsular antigen of Esckrkti coli 08: K50: Hwas shown by glycose analysis, methylation analysis, and one-and two-dimensional 'H and 13C NMR spectroscopy to be composed of repeating linear tetrasaccharide units having the structure: + 3)-@-Rhap-(l + &Y-D-GlcpNAc-(l
Structure of the serine-containing capsular poly-saccharide K40 antigen from Escherichia coli O8:K40:H9
โ Scribed by Thomas Dengler; Barbara Jann; Klaus Jann
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 524 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
The structure of the K40 antigenic capsular polysaccharide (K40 antigen) of E. coli O8:K40:H9 was elucidated by determination of the composition, 1H- and 13C-n.m.r. spectroscopy, periodate oxidation and Smith degradation, and methylation analysis. The K40 polysaccharide consists of [(O-beta-D-glucopyranosyluronic acid)-(1----4)-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-(1----6)-O -(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-(1----4)] repeating units. All of the glucuronic acid residues are substituted amidically with L-serine.
๐ SIMILAR VOLUMES
The structure of the capsular polysaccharide from Escherichia coli 08:KS:H4 has been elucidated, using mainly methylation analysis, Smith degradation, and lD-and 2D-n.m.r. spectroscopy. The polysaccharide, after removal of bound lipid, was found to be composed of repeating units of the linear tetras
The primary structure of the acidic capsular antigen of Escherichia coli 08:K25:H9 was shown by monosaccharide analysis, methyl&on analysis, and by 1D and 2D 'H and 13C NMR spectroscopy to be composed of linear tetrasaccharide repeating units having the structure: + 2) -fi-D-GkpA-( 1 --, 3) -cY-D-Ga
The K16-antigen from E. coli Rk 21510 (O7:K16:H-) is shown to consist of the repeating unit ----2)-beta-D-Ribf-(1----3)-beta-D-Ribf-(1----5)-alpha-Kd op-(2---- of which approximately 33% is O-acetylated at position 3 of the 2-linked ribose.