The structure of the capsular polysaccharide from Escherichia coli 08:KS:H4 has been elucidated, using mainly methylation analysis, Smith degradation, and lD-and 2D-n.m.r. spectroscopy. The polysaccharide, after removal of bound lipid, was found to be composed of repeating units of the linear tetras
Structure of the capsular antigen of Escherichia coli O8:K50:H−
✍ Scribed by M.Ruth Grue; Haralambos Parolis; Lesley A.S. Parolis
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 529 KB
- Volume
- 258
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The primary structure of the acidic capsular antigen of Esckrkti coli 08: K50: Hwas shown by glycose analysis, methylation analysis, and one-and two-dimensional 'H and 13C NMR spectroscopy to be composed of repeating linear tetrasaccharide units having the structure: + 3)-@-Rhap-(l + &Y-D-GlcpNAc-(l + 2)-~-~-hhnp_(l+ 3k-D-ManpNAc_(l * v Pyr W
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The primary structure of the acidic capsular antigen of Escherichia coli 08:K25:H9 was shown by monosaccharide analysis, methyl&on analysis, and by 1D and 2D 'H and 13C NMR spectroscopy to be composed of linear tetrasaccharide repeating units having the structure: + 2) -fi-D-GkpA-( 1 --, 3) -cY-D-Ga
The primary structure of the acidic capsular polysaccharide of Escherichia coli O8:K43:H11 was shown by monosaccharide analysis, methylation analysis, fl-elimination, and by 1D and 2D 1H and 13C NMR spectroscopy to be composed of branched pentasaccharide repeating units with the structure ~4)-=-D-Gl
The structure of the K40 antigenic capsular polysaccharide (K40 antigen) of E. coli O8:K40:H9 was elucidated by determination of the composition, 1H- and 13C-n.m.r. spectroscopy, periodate oxidation and Smith degradation, and methylation analysis. The K40 polysaccharide consists of [(O-beta-D-glucop