The annexed structure of the capsular polysaccharide of Escherichia coli O9:K35:H- (A104a) has been determined by glycose and methylation analysis, base-catalysed degradation, and 1D and 2D NMR spectroscopy on the polysaccharide and the products obtained by bacteriophage-mediated depolymerisation. [
The structure of the capsular polysaccharide of Escherichia coli O8:K43:H11
โ Scribed by Y.M. Choy; G.G.S. Dutton; M. Ruth Leslie; Haralambos Parolis; Lesley A.S. Parolis
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 350 KB
- Volume
- 269
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
โฆ Synopsis
The primary structure of the acidic capsular polysaccharide of Escherichia coli O8:K43:H11 was shown by monosaccharide analysis, methylation analysis, fl-elimination, and by 1D and 2D 1H and 13C NMR spectroscopy to be composed of branched pentasaccharide repeating units with the structure ~4)-=-D-GlcpA-(1-3)-I~ -D-Manp-(1-4)-0 -D-Manp-(1-3)-I~-O-GlcpNAc-(1 -2 ! 1
๐ SIMILAR VOLUMES
The structure of the capsular polysaccharide from Escherichia coli 08:KS:H4 has been elucidated, using mainly methylation analysis, Smith degradation, and lD-and 2D-n.m.r. spectroscopy. The polysaccharide, after removal of bound lipid, was found to be composed of repeating units of the linear tetras
The primary structure of the acidic capsular antigen of Esckrkti coli 08: K50: Hwas shown by glycose analysis, methylation analysis, and one-and two-dimensional 'H and 13C NMR spectroscopy to be composed of repeating linear tetrasaccharide units having the structure: + 3)-@-Rhap-(l + &Y-D-GlcpNAc-(l
The structure of the capsular antigen of E. coli K49 and the oligosaccharides derived from it by partial acid hydrolysis were studied by 1D- and 2D-n.m.r. spectroscopy, g.l.c.-c.i.-mass spectrometry, and methylation analysis. The K49 polysaccharide consists of the repeating unit----4)-beta-D-GlcpA-(
The capsular K11 antigen of Escherichia coli contains glucose, fructose, and phosphate in the molar ratios 2:1:1, and a backbone of -4)-beta-D-glucopyranosyl-(1----4)-alpha-D-glucopyranosyl phosphate-(1----to which beta-D-fructofuranose is linked at position 3 of the beta-D-glucopyranosyl residue. T
The primary structure of the acidic capsular antigen produced by E. coli K84 was shown by glycose and methylation analysis, and by 1D and 2D 1H and 13C NMR studies of the polysaccharide and an oligosaccharide produced by lithium-ethylenediamine degradation of the polysaccharide, to be comprised of l