The primary structure of the acidic capsular polysaccharide of Escherichia coli O8:K43:H11 was shown by monosaccharide analysis, methylation analysis, fl-elimination, and by 1D and 2D 1H and 13C NMR spectroscopy to be composed of branched pentasaccharide repeating units with the structure ~4)-=-D-Gl
Structure and serological properties of the capsular K11 antigen of Escherichia coli O13:K11:H11
โ Scribed by Maria-Luisa Rodriguez; Barbara Jann; Klaus Jann
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 538 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
The capsular K11 antigen of Escherichia coli contains glucose, fructose, and phosphate in the molar ratios 2:1:1, and a backbone of -4)-beta-D-glucopyranosyl-(1----4)-alpha-D-glucopyranosyl phosphate-(1----to which beta-D-fructofuranose is linked at position 3 of the beta-D-glucopyranosyl residue. The fructose, which is the immunodominant sugar of the K11 antigen, is released from the polysaccharide under mild acidic conditions (70 degrees, pH 5.0).
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The primary structure of the acidic capsular antigen of Esckrkti coli 08: K50: Hwas shown by glycose analysis, methylation analysis, and one-and two-dimensional 'H and 13C NMR spectroscopy to be composed of repeating linear tetrasaccharide units having the structure: + 3)-@-Rhap-(l + &Y-D-GlcpNAc-(l
The structure of the capsular polysaccharide from Escherichia coli 08:KS:H4 has been elucidated, using mainly methylation analysis, Smith degradation, and lD-and 2D-n.m.r. spectroscopy. The polysaccharide, after removal of bound lipid, was found to be composed of repeating units of the linear tetras
The primary structure of the acidic capsular antigen of Escherichia coli 08:K25:H9 was shown by monosaccharide analysis, methyl&on analysis, and by 1D and 2D 'H and 13C NMR spectroscopy to be composed of linear tetrasaccharide repeating units having the structure: + 2) -fi-D-GkpA-( 1 --, 3) -cY-D-Ga