The primary structure of the acidic capsular antigen of Escherichia coli 020:K101 was shown by glycose analysis, methylation analysis, and one-and two-dimensional 'H and "C NMR spectroscopy to be composed of repeating branched-tetrasaccharide units having the structure: --f 3)-fi-o-GalpNAc-(1 --f 4
Structural investigation of the capsular polysaccharide of Escherichia coli O101 : K103 : H− using bacteriophage degradation and NMR spectroscopy
✍ Scribed by M.Ruth Grue; Haralambos Parolis; Lesley A.S. Parolis
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Volume
- 262
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
NMR spectroscopy was performed on the depyruvated capsular antigen of E. coli K103 and on the oligosaccharide obtained by depolymerisation of the native polysaccharide with a viral-borne endoglycanase. This capsular polysaccharide is the only one to be co-expressed with O group 101 and joins a small group of unusual capsular polysaccharides which possess pyruvic acid as the only acidic function. The primary structure was shown to be composed of the repeating unit: [formula: see text]
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