Re-investigation of the structure of the capsular polysaccharide of Klebsiella K15 using bacteriophage degradation and inverse-detected NMR experiments
โ Scribed by Haralambos Parolis; Lesley A.S. Parolis; Darryl V. Whittaker
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 576 KB
- Volume
- 231
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
The structure of the capsular polysaccharide from Klebsiella K15 has been re-investigated, principally by 1D and 2D 1H- and 13C-NMR spectroscopy of the oligosaccharide-alditol obtained by depolymerisation of the polysaccharide with a viral-borne endoglycanase followed by borohydride reduction of the isolated repeating oligosaccharide. The capsular polysaccharide was shown to have the repeating unit: [Formula: see text]
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Sugar analysis of the capsular antigen K19 from KlebsieZfa and of the carboxylreduced derivative confirmed its classification into the chemotype containing rhamnose, galactose, glucose, and glucuronic acid residues. Partial acid hydrolysis and phage depolymerization of K19 provided respectively a mo