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Determination of the phospholipase activity of patatin by a continuous spectrophotometric assay

✍ Scribed by M. Jiménez-Atiénzar; J. Cabanes; F. Gandía-Herrero; J. Escribano; F. García-Carmona; M. Pérez-Gilabert

Book ID
107485323
Publisher
Springer-Verlag
Year
2003
Tongue
English
Weight
120 KB
Volume
38
Category
Article
ISSN
0024-4201

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We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion