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A Spectrophotometric Microtiterplate Assay to Determine the Transphosphatidylation Potential of Phospholipase D

✍ Scribed by Martin Dippe; Renate Ulbrich-Hofmann

Book ID
107494593
Publisher
Springer-Verlag
Year
2010
Tongue
English
Weight
320 KB
Volume
87
Category
Article
ISSN
0003-021X

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A Spectrophotometric Assay for the Trans
A Spectrophotometric Assay for the Transphosphatidylation Activity of Phospholipase D Enzyme
✍ Tairo Hagishita; Masanobu Nishikawa; Tadashi Hatanaka πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 77 KB

We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion