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Determination of the degree of in vivo tRNA aminoacylation in yeast cells

✍ Scribed by B. Ehresmann; P. Imbault; J.H. Weil


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
462 KB
Volume
61
Category
Article
ISSN
0003-2697

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As a consequence of the existence of two classes of aminoacyl-tRNA synthetases (aaRSs), we de"ned two types of mutations: g (mutations that do not change the class of the involved amino acids) and u (those which change the class). We have found that the mean chemical distance resulting from g mutati

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The concentration of proteins in solutions or cell-free extracts contnminated by tRNA's or rRNA's can be simply, rapidly, and accurately determined by measuring the absorbance at 2265 and 2345A, two wavelengths where the absorption due to ribonucleic acids is identical so that their interference can