Determination of the degree of in vivo tRNA aminoacylation in yeast cells
β Scribed by B. Ehresmann; P. Imbault; J.H. Weil
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 462 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
As a consequence of the existence of two classes of aminoacyl-tRNA synthetases (aaRSs), we de"ned two types of mutations: g (mutations that do not change the class of the involved amino acids) and u (those which change the class). We have found that the mean chemical distance resulting from g mutati
The concentration of proteins in solutions or cell-free extracts contnminated by tRNA's or rRNA's can be simply, rapidly, and accurately determined by measuring the absorbance at 2265 and 2345A, two wavelengths where the absorption due to ribonucleic acids is identical so that their interference can