A simple assay is described in which small numbers of endothelial cells in culture can be determined by measuring acid phosphatase activity. After removal of the growth medium from cells grown in 96-well culture plates, the cells are lysed in buffer containing the detergent Triton X-100 and the phos
β¦ LIBER β¦
Use of phenolphthalein diphosphate in the determination of phosphatase activities of yeast cells
β Scribed by G.W.F.H. Borst Pauwels
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 457 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Determination of the number of endotheli
β
Daniel T. Connolly; Mary Beth Knight; Nikos K. Harakas; Arthur J. Wittwer; Josep
π
Article
π
1986
π
Elsevier Science
π
English
β 361 KB
Use of permeabilized yeast cells for the
β
Carlos Pascual; Roberto Pascual; ArnoΕ‘t Kotyk
π
Article
π
1982
π
Elsevier Science
π
English
β 187 KB
The Determination of Phenolphthalein in
β
Warren, Alexander T. ;Logun, John E. ;Thatche, Raymond L.
π
Article
π
1950
π
Elsevier
β 197 KB
π 1 views
The determination of phenolphthalein in
β
Frederick, Michael ;Koff, Arnold
π
Article
π
1946
π
Elsevier
β 124 KB
π 1 views
Determination of the degree of in vivo t
β
B. Ehresmann; P. Imbault; J.H. Weil
π
Article
π
1974
π
Elsevier Science
π
English
β 462 KB
Use of Bioluminometry for Determination
β
M. NavrΓ‘til; Z. DΓΆmΓ©ny; V. HronskΓ½; E. Ε turdΔ±Μk; D. Ε mogroviΔovΓ‘; P. Gemeiner
π
Article
π
2000
π
Elsevier Science
π
English
β 71 KB
The technique of bioluminometry was used to determine the biomass concentration of yeast cells immobilized in ionotropic hydrogel beads, including alginate, pectate, and -carrageenan. The method uses determination of ATP extracted from viable cells, the concentration of which is then expressed as th