𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of the 2,4-dinitrophenylhydrazones of urine ketosteroids by thin layer densitometry

✍ Scribed by P. Knapstein; J.C. Touchstone

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
826 KB
Volume
37
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

✦ Synopsis

A double beam scanning spectrodensitometer is used for the determination of DHEA (dehydroepiandrosterone = '3@hydroxyandrost-5-en-I7-one), :and?osterone (3p-hydroxy-ga-androstan-r7-one) and etiocholanolone (go-hydroxy-$3~andr&tanir$one) from urine. After ether solvolysis, purification and reaction with ?,dp+linitro? phenylhydrazine, aliquots are developed, on thin layer plates and sua"ti'~~~~~~~'r"ct'y.

..' ,' " . . ,.. . .

πŸ“œ SIMILAR VOLUMES

Thin-layer chromatography of keto acid 2
Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones
✍ Nakao Ariga πŸ“‚ Article πŸ“… 1972 πŸ› Elsevier Science 🌐 English βš– 334 KB

Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate

Determination of phenobarbital and p-hyd
Determination of phenobarbital and p-hydroxyphenobarbital by densitometry of thin layers
✍ S.S. Levin; M.F. Schwartz; D.Y. Cooper; J.C. Touchstone πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 239 KB

Duriug studies of cytochrome P450 activity in rats by induction with phenobarbital (PB), it became of significance to determine methods of detection and quantitation of the drug and its metabohte, p-hydroxyphenobarbital (PPB). Previous attempts at separation have been carried out by gas chromatograp